Blar i forfatter "Robertsen, Børre"
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Adjuvant activity of fish type I interferon shown in a virus DNA vaccination model
Chang, Chia Jung; Sun, Baojian; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-09)There is a need for more efficient vaccines to combat viral diseases of Atlantic salmon and other farmed fish. DNA vaccines are highly effective against salmonid rhabdoviruses, but have shown less effect against other viruses. In the present work we have studied if type I IFNs might be used as adjuvants in fish DNA vaccines. For this purpose we chose a DNA vaccine model based on the hemagglutin ... -
Analysis of the Atlantic salmon genome reveals a cluster of Mx genes that respond more strongly to IFN gamma than to type I IFN
Robertsen, Børre; Greiner-Tollersrud, Linn; Jørgensen, Lars Gaute (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-09-06)Mx proteins are antiviral GTPases, which are induced by type I IFN and virus infection. Analysis of the Atlantic salmon genome revealed the presence of 9 Mx genes localized to three chromosomes. A cluster of three Mx genes (SsaMx1 – SsaMx3), which includes previously cloned Mx genes, is present on chromosome (Chr) 12. A cluster of five Mx genes (SsaMx4-SsaMx8) is present on Chr25 while one Mx gene ... -
Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals
Sun, Baojian; Greiner-Tollersrud, Linn; Koop, Ben F; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1
Lauksund, Reidunn Silje; Greiner-Tollersrud, Linn; Chang, Chia Jung; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)Infectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic properties against type I IFN induction in Atlantic salmon. Each of the five IPNV genes cloned into an expression vector were tested for the ability to influence activation of the ... -
Infectious salmon anemia virus (ISAV) replication is transiently inhibited by Atlantic salmon type I interferon in cell culture
Svingerud, Tina; Holand, Jenni; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2013-08-20)Infectious salmon anemia virus (ISAV) is a piscine orthomyxovirus, which causes multisystemic disease in farmed Atlantic salmon that may result in large losses. Previous work has suggested that ISAV is able to resist the antiviral state induced in cells by type I interferon (IFN). These studies were, however, mainly based on cytopathic effect (CPE) reduction assays. Here we have investigated the ... -
Microbial danger signals control transcriptional induction of distinct MHC class I L lineage genes in Atlantic salmon
Svenning, Steingrim; Gondek-Wyrozemska, Agata T; van der Wal, Yorick Andreas; Robertsen, Børre; Jensen, Ingvill; Jørgensen, Jorunn B; Edholm, Eva-Stina Isabella (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-11)Antigen processing and presentation by major histocompatibility complex (MHC) molecules is a cornerstone in vertebrate immunity. Like mammals, teleosts possess both classical MHC class I and multiple families of divergent MHC class I genes. However, while certain mammalian MHC class I-like molecules have proven to be integral in immune regulation against a broad array of pathogens, the biological ... -
Protection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmid
Chang, Chia Jung; Robertsen, Camilla; Sun, Baojian; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-07-02)n this work we have tested the in vivo antiviral activity of type I interferons (IFNs) in Atlantic salmon by injecting presmolts intramuscularly (i.m.) with plasmids encoding IFNa1, IFNb or IFNc under the control of a CMV promoter, and measured expression of antiviral genes in organs and protection against infection with infectious salmon anemia virus (ISAV)infection. Allthree IFN plasmids induced ... -
Sequence Analysis of the Genome of Piscine Orthoreovirus (PRV) Associated with Heart and Skeletal Muscle Inflammation (HSMI) in Atlantic Salmon (Salmo salar)
Markussen, Turhan; Dahle, Maria Krudtaa; Tengs, Torstein; Løvoll, Marie Therese; Finstad, Øystein Wessel; Wiik-Nielsen, Christer R.; Grove, Søren; Lauksund, Reidunn Silje; Robertsen, Børre; Rimstad, Espen (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation (HSMI) of farmed Atlantic salmon (Salmo salar). We have performed detailed sequence analysis of the PRV genome with focus on putative encoded proteins, compared with prototype strains from mammalian (MRV T3D)- and avian orthoreoviruses (ARV-138), and aquareovirus (GCRV-873). Amino acid identities were low for ... -
Sequence Analysis of the Genome of Piscine Orthoreovirus (PRV) Associated with Heart and Skeletal Muscle Inflammation (HSMI) in Atlantic Salmon (Salmo salar)
Markussen, Turhan; Dahle, Maria Krudtaa; Tengs, Torstein; Løvoll, Marie Therese; Finstad, Øystein Wessel; Wiik-Nielsen, Christer R.; Grove, Søren; Lauksund, Reidunn Silje; Robertsen, Børre; Rimstad, Espen (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)Piscine orthoreovirus (PRV) is associated with heart- and skeletal muscle inflammation (HSMI) of farmed Atlantic salmon (Salmo salar). We have performed detailed sequence analysis of the PRV genome with focus on putative encoded proteins, compared with prototype strains from mammalian (MRV T3D)- and avian orthoreoviruses (ARV-138), and aquareovirus (GCRV-873). Amino acid identities were low for ... -
Structural and functional characterization of salmon STAT1, STAT2 and IRF9 homologs sheds light on interferon signaling in teleosts
Sobhkhez, Mehrdad; Skjesol, Astrid; Thomassen, Ernst Ivan; Iliev, Dimitar Borisov; Sun, Baojian; Robertsen, Børre; Jørgensen, Jorunn B (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)Mammalian IRF9 and STAT2, together with STAT1, form the ISGF3 transcription factor complex, which is critical for type I interferon (IFN)-induced signaling, while IFNγ stimulation is mediated by homodimeric STAT1 protein. Teleost fish are known to possess most JAK and STAT family members, however, description of their functional activity in lower vertebrates is still scarce. In the present study we ... -
Transcriptome analyses of Atlantic salmon muscle genes induced by a DNA vaccine against salmonid alphavirus, the causative agent of salmon pancreas disease (PD)
Sobhkhez, Mehrdad; Krasnov, Aleksei; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-10-01)Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD) in farmed Atlantic salmon. A previous study showed that vaccination of pre-smolt salmon with a plasmid encoding the structural polypeptide of SAV gave protection against infection and development of PD accompanied by production of antibodies against the virus. In the present work we analyzed transcript responses in the muscle ... -
Transcriptome analysis of plasmid-induced genes sheds light on the role of type I IFN as adjuvant in DNA vaccine against infectious salmon anemia virus
Sobhkhez, Mehrdad; Krasnov, Aleksei; Chang, Chia Jung; Robertsen, Børre (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-11-21)A previous study showed that a plasmid expressing IFNa (pIFNa) strongly enhanced protection and antibody production of a DNA vaccine against infectious salmon anemia virus (ISAV) in Atlantic salmon. The vaccine consisted of a plasmid (pHE) expressing the virus hemagglutinin-esterase as an antigen. To increase the understanding of the adjuvant effect of pIFNa, we here compared transcriptome responses ...